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Center for Research in Biological Systems
University of California, San Diego
9500 Gilman Drive
La Jolla, CA 92093-0608, USA
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Search Results for
GTPase activity
(154 results)
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Still Images
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Z-Stack
Time Series
CIL:13571
NCBI Organism Classification
Homo sapiens
Biological Process
Golgi organization
Cellular Component
Golgi stack
Wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) colocalizes primarily at the Golgi (Giantin) (red) with Helix Pomatia Lectin (HPL) (green) in unstimulated HeLa cells. HPL binds various glycans but the Tn ant...
CIL:13572
NCBI Organism Classification
Homo sapiens
Biological Process
Golgi organization
Cellular Component
Golgi stack
Constitutively active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) colocalizes primarily at the Golgi (Giantin) (red) with Helix Pomatia Lectin (HPL) (green) in unstimulated HeLa cells. HPL binds various g...
CIL:13575
NCBI Organism Classification
Mus musculus
Biological Process
signal transduction
Cellular Component
Golgi stack
Wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) does not block redistribution of Helix Pomatia Lectin (HPL) (green) from Golgi to ER in Src-deficient SYF fibroblasts cotransfected with active Src (SrcE-DsRe...
CIL:13882
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13885). This image shows SPB localization of eGFP-Tem1 (eGFP, g...
CIL:13862
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cytokinesis
Cellular Component
spindle pole body
In tem1Δ cells expressing a Cnm67-Tem1 fusion protein, Cdc15-3HA (red) loads prematurely onto spindle pole bodies during metaphase as determined by spindle morphology (tubulin, green) and nuclear mor...
CIL:13863
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cytokinesis
Cellular Component
spindle pole body
In tem1Δ cells expressing a Bfa1-Tem1 fusion protein, Cdc15-3HA (red) loads prematurely onto spindle pole bodies during metaphase as determined by spindle morphology (tubulin, green) and nuclear morp...
CIL:13881
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
spindle pole body
Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (CIL# 13880) but can also still localize to the SPB (this image). The CAAX motif from Ras2 targets proteins to the cytopl...
CIL:13577
NCBI Organism Classification
Homo sapiens
Biological Process
Golgi organization
Cellular Component
Golgi stack
Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of Helix Pomatia Lectin (HPL) (green) from t...
CIL:13877
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Tem1 (green) localizes to spindle pole bodies (SPB) during metaphase. Control image for CIL# 13876 in which Tem1 loading onto SPBs was severely affected in bfa1∆ cells. DAPI (blue) and DIC (hidden) ...
CIL:13880
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
spindle pole body
Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (this image) but can also still localize to the SPB (CIL# 13881). The CAAX motif from Ras2 targets proteins to the cytopl...
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