Phase contrast image of PtK1 cell (marsupial kidney epithelial cell) injected with Pak1 inhibitory fragment (H83L), X-rhodamine tubulin, and expressing constitutively active Rac(Q61L). Microtubule dyn...

Microtubules in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence i...

Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Fast retrograde flow still occurs in the lamellipodium, but the width of the lamellipodium is re...

Phase contrast of a PtK1 cell (marsupial kidney epithelial cell) injected with Pak1 inhibitory fragment (H83L, 120 uM needle concentration) and X-rhodamine tubulin. Microtubules dynamics in these cell...

Differential interference microscopy (DIC) image of PtK1 cell (marsupial kidney epithelial cell expressing dominant-negative Rac1(T17N). Live cells were used to study microtubule dynamics using X-rhod...

Microtubules in a PtK1 cell injected with the Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were ac...

Actin dynamics in a Rac1(Q61L)-expressing PtK1 cell. Fast retrograde flow occurs in the lamellipodium and slow retrograde flow in the lamellum. The cell was microinjected with X-rhodamine-conjugated a...

Phase contrast image of a PTK-1 cell expressing constitutively active Rac1(Q61L). Live cells were visualized using X-rhodamine tubulin (injected at 1 mg/mL)and eGFP-Rac1(Q61L) (injected into the nucl...

Phase contrast image of control PtK1 cell whose microtubules were visualized by microinjected X-rhodamine tubulin and fluorescence microscopy. This cell corresponds to Figure 1 and Video 1 of J Cel...

Microtubules in a control PtK1 cell at the edge of an epithelial cell island. Few pioneer microtubules rapidly grow into forming protrusions. The cell was microinjected with X-rhodamine-conjugated tub...