Alternate header for print version


Reconstruction
Full resolution image description
Compressed zip file containing the tomographic reconstruction in Analyze 7. 5 (hdr.img/hdr) and IMOD (.rec) format
File format
Analyze 7.5
Volume_dimension
2007, 3006, 150
Animation description
Animation of the computed slices through a tomographic volume of mitochondria at the Node of Ranvier in rat peripheral nerve root

Image 2D
Full resolution image description
Stacked raw data in IMOD format. As stated in the publication, the data was taken at a variety of magnifications. The earliest version obtainable was already cropped and prealigned.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:8141*
Project: P2083
Project name
Morphology of mitochondria in peripheral nerves
Description
Study of morphology of quantitative morphology of mitochondria in the peripheral nerve of the adult rat using 3D electron microscopy
Funding agency
National Institute of Neurological Disorders and Stroke, National Center for Research Resources, National Institute of Diabetes and Kidney Diseases, National Library of Medicine
Leader(s)
Mark H. Ellisman
Guy A. Perkins
Start date
unspecified
End date
05-18-2010
 
Experiment
Experiment ID
8114
Title
Experiment 1
Purpose
Comparison of high pressure freezing and conventional fixation on mitochondrial structure using electron tomography
Experimenter(s)
Guy Perkins
Microscopy product
Microscopy product ID
8141
Instrument
JEOL 4000EX microscope
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
enface
Subject
Species
Rat
Scientific name
Rattus norvegicus
Strain
Sprague Dawley
Age
1 months
Age class
young adult
Tissue section
Anatomical location
spinal root
Microtome
Ultramicrotome
Specimen description
Organ
spinal root
System
peripheral nervous system
Structure
mitochondrion
Imaging parameters
Type
Electron microscopy product
Magification
0
Specimen preparation
Protocol used
Chemical fixation with 2% paraformaldehyde and 2.5% glutaraldehyde via vascular perfusion in 0.15M cacodylate buffer (pH 7.4) at 37 degrees C Neuronal sections cut to a thickness of 300-500 nm were taken on 100 mesh copper clamshell grids. The sections on the grid were stained for 30 minutes to 60 minutes in 2% aqueous uranyl acetate then approximately 15 min in lead salts. 20nm fiducial markers were placed on the specimen.
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees