Alternate header for print version


Reconstruction
Display image description
Optical section series through adjacent hippocampal astrocytes injected with Lucifer Yellow (green) and Alexa568 (red) respectively, and immunolabeled for GFAP (blue), imaged with confocal microscopy

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:62*
Project: P1230
Project name
Astrocyte Development
Description
Postnatal development of protoplasmic astrocytes
Funding agency
NIH
Leader(s)
Eric Bushong
Collaborator(s)
Maryann Martone
Mark Ellisman
Start date
02-01-2002
End date
unspecified
 
Experiment
Experiment ID
25
Title
Morphology of astrocytes in 1 week old hippocampus
Purpose
Examine the morphology of 1 week old astrocytes
Experimenter(s)
Eric Bushong
Microscopy product
Microscopy product ID
62
Instrument
Biorad Radiance 2000 Confocal
Microscopy type
confocal
Product type
optical section series
Image basename
1wk-both6
Spatial Axis Image Size Pixel Size
X 1024px 0.056061 µm
Y 1024px 0.056061 µm
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Group by
NA
Treatment
NA
Age
1 weeks
Age class
juvenile
Tissue section
Anatomical location
hippocampus
Microtome
vibratome
Tissue product storage
coverslipped
Thickness
100 µm
Specimen description
Organ
brain
System
central nervous system
Cell type
protoplasmic astrocyte
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens
Nikon
Lens magnification
X
Numerical aperture
1.4
Refractive index
1.5
Imaging product type
Type
Through focus
Z step
.2 um