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Reconstruction
Display image description
Single computed slice through a tomographic reconstruction of a mitochondria from a rod photoreceptor in the mouse retina.
Full resolution image description
Tar file containing tomographic volume .img and .hdr files in Analyze 7.5 format
Volume_dimension
736, 1010, 125
Volume scale
0.0024, 0.0024, 0.0024
Animation description
Animation through the slices of a tomographic reconstruction of a mitochondria from a rod photoreceptor in the mouse retina.

Image 2D
Display image description
Single tilt image (zero degree tilt) of a mitochondrion from a retinal cone cell from a 0.3 um section
Full resolution image description
Tar file containing cropped and aligned tilt images plus fiducial mark files.
Animation description
Aligned and cropped tilt images of a mitochondrion from a retinal cone cell

Segmentation
Display image description
Manual tracing of mitochondrial membrane structures using Xvoxtrace and then surfaced using Synu. Each crista was segmented individually

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:54*
Project: P1209
Project name
Cone and Rod mitochondria
Description
In situ structures of mitochondria in rods and cones
Leader(s)
Don Fox
University of Houston
Collaborator(s)
Guy Perkins
Start date
09-01-2001
End date
09-01-2001
 
Experiment
Experiment ID
33
Experiment date
07-01-2001
Title
Cone and rod mitochondria: electron tomography
Purpose
electron tomography of cone mitochondria
Experimenter(s)
Guy Perkins
Microscopy product
Microscopy product ID
54
Instrument
JEOL4000EX
Microscopy type
IVEM
Product type
single tilt
Image basename
cone1
Spatial Axis Image Size Pixel Size
X 1024px 0.00224 µm
Y 1024px 0.00224 µm
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/6
Treatment
none
Age
21 days
Age class
young adult
Tissue section
Anatomical location
retina
Microtome
ultramicrotome
Tissue product storage
Fox WT Durcupan400
Thickness
300 nm
Specimen description
Organ
eye
System
central nervous system
Structure
mitochondrion
Cell type
photoreceptor/cone
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
40000
Accelerating voltage
400 KeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees


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