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Reconstruction
Display image description
Tomographic reconstruction of a spiny dendrite from mouse hippocampus stained by double impregnation with copper and lead salts and imaged using most probable loss energy filtering (see Bouwer et al., 2004). This staining technique also fills membrane bound organelles such as synaptic vesicles with electron dense reaction product.
Full resolution image description
files were aligned in IMOD and reconstructed using TxBr with quadratic corrections

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:51*
Project: P1172
Project name
MPL pilot data
Description
tests for most probable loss tomography on the 3200
Funding agency
NIH IVEM
Leader(s)
James Bouwer
Collaborator(s)
Mark Ellisman
Steven Peltier
Mason Mackey
Start date
04-01-2004
End date
unspecified
 
Experiment
Experiment ID
31
Title
Copper Lead Specimens
Purpose
To provide a test specimen for MPL imaging using copper lead impregnation
Experimenter(s)
Ying Jones
Microscopy product
Microscopy product ID
51
Instrument
JEM 3200 EF
Microscopy type
IVEM
Product type
single tilt tomography
Image basename
spin_filt
Spatial Axis Image Size Pixel Size
X 4000px
Y 4000px
Subject
Species
mouse
Scientific name
mus musculus
Strain
Unspecified
Age class
unspecified
Tissue section
Anatomical location
hippocampus
Microtome
ultramicrotome
Thickness
1 µm
Specimen description
Organ
brain
System
central nervous system
Structure
spiny dendrite, synaptic vesicles
Cell type
pyramidal neuron
Imaging parameters
Type
Electron microscopy product
Recording medium
Tietz 4 K CCD
Magification
4000
Energy filter slit
most probable loss with 35 eV slit
Accelerating voltage
300 KeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
1 degrees


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