Alternate header for print version


Reconstruction
Display image description
Large scale mosaic of rat thalamus reconstructed through automated acquisition and stitching of over 447 tiles, triple labeled for GFAP (Red), Pannexin 1(Green) and DAPI (Blue), and imaged using laser scanning confocal microscopy. The contrast and brightness was adjusted for display purposes.
Full resolution image description
Large scale mosaic of rat thalamus reconstructed through automated acquisition and stitching of over 447 tiles, triple labeled for GFAP (Red), Pannexin 1(Green) and DAPI (Blue), and imaged using laser scanning confocal microscopy. The contrast and brightness was adjusted for display purposes.
File format
PPM
Volume_dimension
18798, 18965, 1
Volume scale
0.239, 0.239, 2.8

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:48338*
Project: P20002
Project name
Pannexin expression in the rodent brain
Description
A neuroinformatics approach to pannexin expression, localization, and interaction with other brain proteins. This research aims to examine brain tissue at high resolution and, in combination with developing neuronformatics tools, to probe for binding partners and signalling partners to investigate the pannexin interactome . Data submissions for this project will consist of wide-scale high resolution confocal mosaic images of triple-labeled whole mouse brain sections. Pannexin 1 expression will be compared to that of pannexin 2 as well as the connexins. Other putative interaction partners of interest include the P2 purinergic receptors and components of the cytoskeleton.
Funding agency
NIH F32GM092457
Leader(s)
Angela Cone
Start date
08-01-2009
End date
08-01-2009
 
Experiment
Experiment ID
39127
Title
Diatheva Panx1 Ab in Rat Cerebellum
Purpose
Examine Panx1 Ab labelling
Experimenter(s)
Angela Cone
Microscopy product
Microscopy product ID
48338
Instrument
Olympus IX81
Microscopy type
LASER SCANNING CONFOCAL
Product type
MOSAIC
Image basename
04132011 CkDiatheva Ab in Female Rat Thalamus #2
Spatial Axis Image Size Pixel Size
X 1024px 0.239 um/pixels
Y 1024px 0.239 um/pixels
Y 1px
Subject
Species
Rat
Scientific name
NA
Strain
Sprague Dawley Rat
Age
8 weeks
Age class
Juvenile
Tissue section
Anatomical location
Cerebellum
Microtome
Leica vibratome
Tissue product storage
cryoprotectant solution at -20C
Thickness
50 µm
Specimen description
Organ
brain
System
Central nervous system
Structure
None
Cell type
astrocyte
Imaging parameters
Type
Light microscopy product
Immersion medium
oil
Mounting medium
gelvatol
Lens
Olympus 40X oil
Lens magnification
X
Numerical aperture
1.3
Notes
acone
Imaging product type
Type
Mosaic
Description
04132011 CkDiatheva Ab in Female Rat Thalamus #2