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Reconstruction
Display image description
Single image through a serial section electron microscopic reconstruction of the cell body of a cortical pyramidal neuron from a biopsy sample obtained from the cortex of a patient with Alzheimer's disease
Full resolution image description
Aligned stack of serial section images in IMOD MRC format. Images were aligned through manual assignment of fiducial marks, followed by non-linear alignment in IMOD using Midas, available in IMOD.
File format
MRC
Volume_dimension
4917, 6340, 69
Volume scale
0.04, 0.04, 0.25

Image 2D
Display image description
Electron micrograph of the cell body of a 0.25 um thick section through a cortical pyramidal neuron from the brain of a patient with Alzheimer's disease. The specimen was prepared from biopsy material obtained several decades ago.
Full resolution image description
Tar file containing the unaligned scanned images at full resolution in tiff format

Segmentation
Display image description
Alzheimer's neuron
Segmentation file description
Tar file containing the trace file in Jinx format and the surfaced objects in IV format. Also included is an animation of the surfaced objects in mpeg format. Finally, a synu file viewable on unys of the surfaced objects is included.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3716*  Cite 
Project: P1700
Project name
Alzheimer neuron reconstructions
Description
Reconstruction of cortical neurons from biopsy material obtained from Alzheimers disease patients
Funding agency
National Institutes of Health
Leader(s)
Mark Ellisman
Collaborator(s)
Rama Ranganathan
Thomas J. Deerinck
Stephen J. Young
David Hessler
and Robert D. Terry
Start date
unspecified
End date
unspecified
 
Experiment
Experiment ID
3448
Experiment date
01-01-1987
Title
Alzheimer reconstruction
Purpose
Reconstruction of Alzheimers neurons using serial section EM
Experimenter(s)
Tom Deerinck
Microscopy product
Microscopy product ID
3716
Instrument
Jeol 100CX
Microscopy type
TEM
Product type
SERIAL SECTION
Image basename
ALZ301_1
Subject
Species
human
Scientific name
homo sapiens
Strain
sapiens
Group by
disease status
Treatment
patients diagnosed with Alzheimers disease
Age class
aged
Tissue section
Anatomical location
cerebral cortex
Microtome
ultramicrotome
Thickness
0.25 µm
Specimen description
Organ
brain
System
central nervous system
Structure
soma
Cell type
Neocortex pyramidal cell
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
3300
Accelerating voltage
100 KeV
Imaging product type
Type
Serial section
Z resolution
0.25 µm