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Reconstruction
Display image description
Electron micrograph of of spiny dendrites from medium spiny neurons in a dopamine transporter knock out mouse using diolistic labeling, imaged using ultra high voltage electron microscopy.
Full resolution image description
Tar file containing the the IMOD .hdr/.img files for the of the electron tomograph (tg6dkg14a_an_sub.hdr/.img)
Volume_dimension
2689, 4006, 500
Animation description
Animation of aligned electron microscopic tilt series of a spiny dendrite from medium spiny neurons in a dopamine transporter knock out mouse using diolistic labeling, imaged using ultra high voltage electron microscopy. Tilt series was obtained at 2 degree increments through +/- 66 degrees of tilt. Note: contrast has been reversed so that labeled dendrite appears bright and unstained tissue appears dark.

Image 2D
Display image description
Electron micrograph of a spiny dendrite from medium spiny neurons in a dopamine transporter knock out mouse using diolistic labeling, imaged using ultra high voltage electron microscopy.
Full resolution image description
Tar file containing IMOD files (tg6dkg14a.com/.log/.st/.preali/.fid/.rawtlt) used for the alignment and the original tiff images (in the TIFF folder in the format tg6dkg14a000.tif.gz)
Animation description
Animation of aligned electron microscopic tilt series of a spiny dendrite from medium spiny neurons in a dopamine transporter knock out mouse using diolistic labeling, imaged using ultra high voltage electron microscopy. Tilt series was obtained at 2 degree increments through +/-66 degrees of tilt.

Segmentation
Display image description
dendritic shaft and spines
Segmentation file description
Tar file containing Xvoxtrace files (tg6dkg14a.trace and viewdata), Synu files (shaft.synu, Spine_01.synu, Spine_02.synu, etc.) and the Analyze volume (tg6dkg14a_res2.img/.hdr) for the segmentation. Also includes the image map file in tiff format (tg6dkg14a.imagemap.tiff).

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3652*  Cite 
Project: P1207
Project name
Correlative microscopic characterization of dendritic spines in a transgenic mouse model of hyperdopaminergia: The dopamine transporter knockout mouse
Description
Multiscale characterization of DAT KO transgenic mouse
Funding agency
NIH
Leader(s)
Diana Price
Collaborator(s)
Aki Laakso
Michele Cyr
Maryann Martone
Naoko Yamada
Andrea Thor
Monica Berlanga
Start date
01-01-2003
End date
unspecified
 
Experiment
Experiment ID
3414
Title
P1207 Experiment 6
Purpose
Tomographic reconstruction of spiny dendrites from medium spiny neurons in a dopamine transporter knock out mouse using diolistic labeling
Experimenter(s)
Diana Price and Andrea Thor
Microscopy product
Microscopy product ID
3652
Instrument
Hitachi 3MeV UHVEM
Microscopy type
UHVEM
Product type
SINGLE TILT
Image basename
tg6dkg14a
Subject
Species
mouse
Scientific name
mus musculus
Strain
B6;129-/Slc6a3 tm2Mca
Group by
genetic manipulation
Treatment
dopamine transporter knock out
Age class
adult
Tissue section
Anatomical location
ventral lateral striatum Cell 060504d
Microtome
ultramicrotome
Thickness
4 µm
Specimen description
Organ
brain
System
central nervous system
Structure
spiny dendrite
Cell type
medium spiny neuron
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
3000
Accelerating voltage
3 MeV
Specimen preparation
Protocol used
Add diolistics protocol
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-66 degrees
Max range
66 degrees
Tilt increment
2 degrees