Alternate header for print version


Reconstruction
Display image description
a single slice from the slice-by-slice reconstructed volume of a Caulobacter Crescentus specimen. This particular slice is taken from Z slice # 142.
Full resolution image description
a .tar file containing the final reconstructed slice-by-slice volume of a Caulobacter Crescentus specimen. Volumes are in an IMOD .mrc file format.
Volume_dimension
2242, 3340, 280
Animation description
a .mpg file of the slice by slice reconstructed volume of a Caulobacter Cresecentus specimen.

Image 2D
Display image description
Caulobacter Crescentus specimen from the tilt series. This particular image was taken from the 0 degree tilt.
Full resolution image description
all IMOD processing files needed for a slice by slice volume reconstruction. Images, and consequently the original mrc .st file have not been binned and are their original 4k by 6k file sizes.
Animation description
a .mpg movie file of the tilt series taken of a caulobacter crescentus specimen. Movie starts at the zero degree tilt, and then rocks back and forth before moving back to the zero degree tilt.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:3566*  Cite 
Project: P1220
Project name
Caulobacter
Description
Tomography of Caulobacter crescentus
Funding agency
Department of Energy
Leader(s)
Lucy Shapiro
Harley McAdams
Collaborator(s)
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Start date
07-01-2003
End date
unspecified
 
Experiment
Experiment ID
3374
Experiment date
05-12-2004
Title
Tomographic reconstruction of caulobacter
Purpose
Produce a series of tomographic data of the normal morphology of Caulobacter Crescentus using rapid freezing methods.
Experimenter(s)
Patrick Viollier
Tom Deerinck
Microscopy product
Microscopy product ID
3566
Instrument
JEOL 4000
Microscopy type
IVEM
Product type
SINGLE TILT
Image basename
p1220_b39_e5_2bin4
Spatial Axis Image Size Pixel Size
X 2242px 1 nm/pixels
Y 3340px 1 nm/pixels
Subject
Species
Caulobacter
Scientific name
Caulobacter crescentus
Strain
3724
Treatment
None
Age class
not applicable
Tissue section
Anatomical location
N/A
Thickness
0.5 µm
Specimen description
Cell type
Caulobacter crescentus
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
25000
Accelerating voltage
400 kV
Specimen preparation
Protocol used
1. Cocktail - 2% Osmium tetroxide, 0.5% urynal acetate in anhydrous acetone2. Time -T1: -90 Celsius for 72 hoursS1: 2 Celsius per hour for 15 hoursT2: -60 Celsius for 12 hoursS2: 5 Celsius per hour for 12 hoursT3: -20 Celsius HoldLeica FSU
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-66 degrees
Max range
68 degrees
Tilt increment
2 degrees