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Reconstruction
Display image description
Tomographic reconstruction of a portion of medium spiny neuron in a 4 um thick section. This data set is poorly aligned due to a lack of adquate fiducial markers. This volume is an example of a poor reconstruction
Full resolution image description
Volume reconstruction of osaka4 in Analyze format; volume has been sigma filtered using Analyze
Volume_dimension
740, 568, 350
Volume scale
0.007, 0.007, 0.007

Image 2D
Display image description
Single tilt image (zero degree tilt) through a 4 um thick section of spiny dendrite from a medium spiny neuron that was injected with Lucifer Yellow then photooxidized
Full resolution image description
tar file contains the original tilt images compressed using the compress command; osaka4.????.f.Z. Unfortunately; the original fiducial mark file was not saved.
Animation description
Aligned and cropped tilt images from a striatal medium spiny neuron that was injected with Lucifer Yellow and then photooxidized. No fiducial marks were available and so the alignment and subsequent reconstruction are poor. The quality of the microscopic images is fine though, so they can be re-aligned.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:27*
Project: P1119
Project name
Correlated Microscopy of Dendritic Spines
Description
Measurements of spine parameters using light microscopy and electron tomography
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Naoko Yamada; Gordun Arbuthnott; Cali Ingham; Stephen Young
Start date
01-01-1992
End date
unspecified
 
Experiment
Experiment ID
9
Title
Tomography of spiny dendrite at 3 MeV
Purpose
whether 3MeV can be used for tomography of very thick sections
Experimenter(s)
Naoko Yamada
Microscopy product
Microscopy product ID
27
Instrument
Hitachi UHVEM
Microscopy type
UHVEM
Product type
single tilt
Image basename
osaka4
Spatial Axis Image Size Pixel Size
X 1024px 0.007 µm
Y 1024px 0.007 µm
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Age class
adult
Tissue section
Anatomical location
neostriatum
Microtome
ultramicrotome
Thickness
4 µm
Specimen description
Organ
brain
System
central nervous system
Structure
spiny dendrite
Cell type
medium spiny neuron
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
10000
Accelerating voltage
3 MeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-66 degrees
Max range
70 degrees
Tilt increment
2 degrees


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