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Reconstruction
Full resolution image description
Volume reconstruction of selectively stained spiny dendrite
Volume_dimension
491, 680, 281
Volume scale
0.008, 0.008, 0.008

Segmentation
Display image description
manual segmentation using Xvoxtrace; surfaced with Synu using Nuage algorithm; tarfile contains all synu objects listed; the associated Viewdata file required by Synu for viewing; and Xvoxtrace file (oka4.trace) used to contour the objects.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:24*
Project: P1119
Project name
Correlated Microscopy of Dendritic Spines
Description
Measurements of spine parameters using light microscopy and electron tomography
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Naoko Yamada; Gordun Arbuthnott; Cali Ingham; Stephen Young
Start date
01-01-1992
End date
unspecified
 
Experiment
Experiment ID
9
Title
Tomography of spiny dendrite at 3 MeV
Purpose
whether 3MeV can be used for tomography of very thick sections
Experimenter(s)
Naoko Yamada
Microscopy product
Microscopy product ID
24
Instrument
Hitachi 1MeV HVEM
Microscopy type
HVEM
Product type
single tilt
Image basename
oka4
Spatial Axis Image Size Pixel Size
X 1024px 0.008 µm
Y 1024px 0.008 µm
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Age class
adult
Tissue section
Anatomical location
neostriatum
Microtome
ultramicrotome
Thickness
2 µm
Specimen description
Organ
brain
System
central nervous system
Structure
spiny dendrite
Cell type
medium spiny neuron
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
10000
Accelerating voltage
1 MeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees


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