Alternate header for print version


Reconstruction
Display image description
Tomograhic reconstruction of portion of spiny dendrite from a medium spiny neuron from mouse nucleus accumbens. Neuron was filled with Lucifer Yellow and then photooxidized. To view correlated light microscopic data, click View Correlated Data
Full resolution image description
Tar file containing the tomographic volume in Analyze 7.5 format (acc1_2ma.img/hdr).
Volume_dimension
360, 920, 191
Volume scale
0.02, 0.02, 0.02
Animation description
maximum intensity projection through spiny dendrite rotated in 5 degrees increments on the y axis. Contrast is reversed so that the stained dendrite appears bright against a dark background

Image 2D
Display image description
Zero tilt image from a single tilt series through a 2 ?m thick section of a medium spiny neuron that has been intracellularly injected and photooxidized
Full resolution image description
Tar file containing full sized unaligned tilt images in Suprim format (tomo1.*.f) along with the fiducial mark file used to align them (tomo1.fido) and the aligned, cropped images used for the reconstruction (tomo1.*.crop). An animation of the aligned, cropped images may be viewed by clicking on the animation link. Note the basename for the 2D data files (tomo1) is different than the basename for the volume (acc1_2ma).
Animation description
Animation through the aligned tilt series

Segmentation
Display image description
Manual segmentation of dendritic spines using Xvoxtrace 2.11 from tomographic volume of spiny dendrite from mouse nucleus accumbens. Each spine was segmented as a separate object. Spines were surfaced and rendered using Synu. The display image shows a rendering of the dendritic spines (multiple colors) and the dendritic shaft (gray). The dark blue face on the dendritic shaft represents the shorn face of the dendrite at the volume surface.
Segmentation file description
Tar file containing the segmented spines and dendritic shaft from the tomographic volume. Tarfile contains objects surfaced with Synu (*.synu), the associated Viewdata file required by Synu for viewing; and Xvoxtrace file (acc1_2ma.trace) used to contour the objects. Clicking on "All details" link in the segmentation window will return a list of segmented objects and their descriptions.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:14*
Project: P0000
Project name
Mouse BIRN test data
Description
NCMIR test data for Mouse BIRN
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Eric Bushong
Start date
09-01-2001
End date
unspecified
 
Experiment
Experiment ID
1
Experiment date
12-13-2001
Title
Intracellular Injection of Nucleus Accumbens Neuron
Purpose
to obtain multi resolution data for Mouse BIRN
Experimenter(s)
Eric Bushong
Microscopy product
Microscopy product ID
14
Instrument
JEOL4000 IVEM
Microscopy type
IVEM
Product type
single tilt
Image basename
ACC1_2ma
Spatial Axis Image Size Pixel Size
X 1024px
Y 1024px
Subject
Species
mouse
Scientific name
mus musculus
Strain
C57BL/6
Treatment
none
Age
2 months
Age class
adult
Tissue section
Anatomical location
nucleus accumbens
Microtome
ultramicrotome
Thickness
2 µm
Specimen description
Organ
brain
System
central nervous system
Structure
spiny dendrite
Cell type
medium spiny neuron
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
3000
Accelerating voltage
400 KeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees
Imaging product type
Type
Stereo pairs
Description
stereopairs_desc
Stereo angles
0



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