Alternate header for print version


Reconstruction
Display image description
Tomographic reconstruction of stained Purkinje cell dendrite from rat cerebellum. Maximum intensity projection through the volume, with constrast reversed so that dendrite appears bright against background
Full resolution image description
Pccor10_inv is an unprocessed volume with the contrast reversed (spiny dendrite appears bright)
Volume_dimension
200, 590, 80
Volume scale
0.026, 0.026, 0.026

Image 2D
Display image description
Zero tilt image from a single tilt series through a 2 um thick section of a Purkinje cell dendrite that has been intracellularly injected and photooxidized
Full resolution image description
This file contains the original tilt images; pccor10.????.f.Z; compressed.To uncompress use the uncompress command in Unix; Also contains the aligned and cropped tilt images; pccor10.????.crop.The file containing the fiducial marks for the alignment
Animation description
Animation through the aligned tilt series

Segmentation
Display image description
manual segmentation using Xvoxtrace; surfaced with Synu using Nuage algorithm; tarfile contains all synu objects listed; the associated Viewdata file required by Synu for viewing; and Xvoxtrace file (pccor10mm.trace) used to contour the objects.
Segmentation file description
Tar file containing original trace file (MM.trace) with manual contours produced with Xvoxtrace and the surface renderings of the dendritic shaft (shaft.synu) and the individual dendritic spines (sp*.synu) in Synu format, along with the Viewdata file required for viewing with Synuview.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:13*
Project: P1119
Project name
Correlated Microscopy of Dendritic Spines
Description
Measurements of spine parameters using light microscopy and electron tomography
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Naoko Yamada; Gordun Arbuthnott; Cali Ingham; Stephen Young
Start date
01-01-1992
End date
unspecified
 
Experiment
Experiment ID
10
Title
spiny dendrite
Purpose
how well dendritic spines can be detected and measured using LM
Experimenter(s)
Naoko Yamada
Microscopy product
Microscopy product ID
13
Instrument
JEOL4000 IVEM
Microscopy type
IVEM
Product type
single tilt
Image basename
pccor10
Spatial Axis Image Size Pixel Size
X 1024px
Y 1024px
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Treatment
none
Age class
adult
Tissue section
Anatomical location
cerebellum
Microtome
ultramicrotome
Thickness
2 µm
Specimen description
Organ
brain
System
central nervous system
Structure
spiny dendrite
Cell type
Purkinje neuron
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
3000
Accelerating voltage
400 KeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-60 degrees
Max range
60 degrees
Tilt increment
2 degrees


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