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Reconstruction
Display image description
Maximum intensity projection of a tomographic reconstruction of stained dendrite from rat neostriatum in a 4 um section.
Volume_dimension
270, 586, 154
Volume scale
0.022, 0.022, 0.022

Image 2D
Display image description
Zero tilt image from a single tilt series through a 4 ?m thick section of a medium spiny neuron dendrite that has been intracellularly injected and photooxidized
Full resolution image description
Tar file containing unaligned compressed tilt images (*.f.gz) and the aligned, cropped images (*.crop) in Suprim format. The fiducial mark file (osaka3.fido) and origin file (osaka3.fido.origin) used to align the tilt images using the program Xfido are also included.
Animation description
Animation through the aligned tilt series

Segmentation
Display image description
manual segmentationi using Xvoxtrace; surfaced with Synu
Segmentation file description
Tar file containing original trace file with manual contours (osaka3.trace) produced by Xvoxtrace v 2.9 and the surface renderings of the dendritic shaft (shaft.synu) and the population of dendritic spines (spine.synu) in Synu format, along with the Viewdata file required for viewing with Synuview.

License
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

CCDB:12*
Project: P1119
Project name
Correlated Microscopy of Dendritic Spines
Description
Measurements of spine parameters using light microscopy and electron tomography
Funding agency
NIH
Leader(s)
Maryann Martone
Collaborator(s)
Naoko Yamada; Gordun Arbuthnott; Cali Ingham; Stephen Young
Start date
01-01-1992
End date
unspecified
 
Experiment
Experiment ID
9
Title
Tomography of spiny dendrite at 3 MeV
Purpose
whether 3MeV can be used for tomography of very thick sections
Experimenter(s)
Naoko Yamada
Microscopy product
Microscopy product ID
12
Instrument
Hitachi UHVEM
Microscopy type
UHVEM
Product type
single tilt
Image basename
osaka3
Spatial Axis Image Size Pixel Size
X 1024px
Y 1024px
Subject
Species
rat
Scientific name
rattus norvegicus
Strain
Sprague Dawley
Age class
adult
Tissue section
Anatomical location
neostriatum
Microtome
ultramicrotome
Thickness
4 µm
Specimen description
Organ
brain
System
central nervous system
Structure
spiny dendrite
Cell type
medium spiny neuron
Imaging parameters
Type
Electron microscopy product
Recording medium
film
Magification
3000
Accelerating voltage
3 MeV
Imaging product type
Type
Single tilt
Description
singlet_desc
Min range
-66 degrees
Max range
70 degrees
Tilt increment
2 degrees


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