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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9971*  Cite 
Description

Normal Rat Kidney (NRK) cells grown in culture expressing Galactosyl Transferase-YFP (GalT-YFP) and p58-CFP. This file is the CFP time series demonstrating the organization of the early secretory pathway (Golgi and intermediate compartment). Images were collected on a Zeiss Spinning Disk confocal as a single z slice over time with CFP imaged using 445ht and YFP imaged using 515ht and a 63X oil objective. Images were collected every 2 minutes. Exposure time: 515 was 50ms; 445 was 200ms: for both channels, the gain was 3, the camera intensification was 200, the laser neutral density was 10.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
permanent cell line cell
Cell Line
NRK
Kidney
Cellular Component
Golgi apparatus
endoplasmic reticulum-Golgi intermediate compartment
Biological Context
Biological Process
constitutive secretory pathway
Attribution
Names
Prasanna Satpute-Krishnan
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9971
Archival Resource Key (ARK)
ark:/b7295/w9cil9971
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
ECFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.174µm
Y 512px 0.174µm
Time 120 seconds