Licensing

Pd
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Description

Non-contractile stalk showing the striated nature of the fibrous “straws.” TEM taken on 6/12/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,570X. Bar = 2µm. A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis.There is a high resolution version of this image in the library (CIL:39169) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Biological Sources

NCBI Organism Classification
Opercularia [NCBITaxon:168247]
Opercularia coarctata
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component
extracellular region
fibril

Biological Context

Biological Process
fibril organization
extracellular stalk organization

Attribution

Name
Richard Allen
Link
http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type
transmission electron microscopy (TEM)
illumination by electrons
Imaging Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop
Data Qualifiers
processed data
suitable for spatial measurements

Dimensions

Spatial Axis Image Size Pixel Size
X 2740px ——
Y 3742px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.