Licensing
Description
Image of the pellicle of Opercularia coarctata. Indentations of the plasma membrane penetrate the alveolar membranes end as coated pits. These pits are also known as pellicular pores are homologous to parasomal sacs of other ciliates. The surface ridges contain an electron opaque rod in the cytosol of unknown composition, seen here in cross section. TEM taken on 6/11/69 by R. Allen with Philips 300 operating at 60kV. Neg. 30,000X. Bar = 0.25µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7336) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Opercularia [NCBITaxon:168247]
- Opercularia coarctata
- Cell Type
- cell by organism
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cell Line
- Cambridge Culture Collection, Cambridge, England, # LB1657/1
- Cellular Component
- GO:0007009
- clathrin coat of coated pit
- alveolar membranes
- rods of unknown material
Biological Context
- Biological Process
- plasma membrane organization
- clathrin coat assembly
- cytoskeletal anchoring at plasma membrane
- cytoskeletal organization
- Molecular Function
- clathrin binding
Attribution
- Name
- Richard Allen (University of Hawaii)
- Link
- http://www5.pbrc.hawaii.edu/allen/
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- detection of electrons
- film
- Parameters Imaged
- electron density
- Source of Contrast
- stain with broad specificity
- Visualization Methods
- stain with broad specificity
- osmium tetroxide
- uranyl salt
- lead salt
- Processing History
- recorded image
- film
- Print from negative scanned to Photoshop.
- Data Qualifiers
- processed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- microtome-sectioned tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 2753px | —— |
| Y | 3624px | —— |