The Cell: An Image Library

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

Comments:

Add to Photobox

CIL:9706^*

Description

A high resolution image of the transition from basal bodies to cilia within a portion of one of the oral membranelles of Euplotes. Parasomal sacs occur between the rows of basal bodies although these endocytic ports are not as evident in Euplotes as in other ciliates. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 7/29/67 by R. Allen with Philips 200. Neg. 28,000X. The raw negative was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Euplotes sp.
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Euplotes sp. from Carolina Biological Supply
Cellular Component: cilium axoneme; axonemal microtubule; microtubule basal body; axonemal dynein complex; adoral zone of membranelles; plasma membrane; clathrin adaptor complex

Biological Context

Biological Process: ciliary or flagellar motility; ciliary cell motility; membranelle orgnization
Molecular Function: structural molecule activity

Attribution

Name: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3810px	0.7nm
Y	4723px	0.7nm

*CIL – Cell Image Library accession number. Please use this to reference an image.