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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9701*  Cite 

Oblique section of the Coleps contractile vacuole pore revealing the supporting microtubules and adjacent fibrous layer. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/4/69 by R. Allen with Philips 300. Neg. 25,100X. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:9255) is available for quantitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Coleps hirtus
Cell Type
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component
contractile vacuole pore
microtubule associated complex
plasma membrane
actin cytoskeleton
Biological Context
Biological Process
water homeostasis
microtubule cytoskeleton organization
microfibril organization
Molecular Function
control of water regulation
Richard Allen
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
print from negative was scanned for photoshop
Spatial Axis Image Size Pixel Size
X 3000px ——
Y 2366px ——