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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9699*  Cite 
Description

The contractile vacuole of Coleps hirtus. The filled CV is continuous with tubules of the smooth spongiome. The tubular nature of mitochondrial cristae is particularly evident in this micrograph. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/2/69 by R. Allen with Philips 300. Neg. 16,000X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:9252) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Coleps hirtus
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component
contractile vacuole
smooth endoplasmic reticulum membrane
mitochondrion
mitochondrial outer membrane
mitochondrial inner membrane
tubular cristae
cortical membranes
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL9699
Archival Resource Key (ARK)
ark:/b7295/w9cil9699
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3000px ——
Y 2410px ——