The Cell: An Image Library

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

Comments:

Add to Photobox

CIL:9694^*

Description

Surface of Coleps hirtus. Alveoli have longitudinal margins along the ciliary rows as well as lateral margins spaced at intervals of one or two basal bodies along the rows. Margins of adjacent alveoli form septa and pores pass through both the lateral and longitudinal septa. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/24/69 by R. Allen with Philips 300 operating at60kV. Print from negative scanned for Photoshop. Neg. 5,000X. Bar = 1µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image is available at (CIL:38830) and can be used for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Coleps hirtus
Cell Type: eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component: plasma membrane; alveolar membranes; membrane pores; axoneme; axonemal microtubule

Biological Context

Biological Process: cell membrane detail; alveoli detail; ciliary or flagellar motility
Molecular Function: cell homeostasis

Attribution

Name: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.

Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3000px	——
Y	2228px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.