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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9171*  Cite 

Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart) Timepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart) Timepoint3: 238 seconds (11 Z-slices at 1 um apart) Timepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point) Images were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
permanent cell line cell
epithelial cell
Cell Line
Cellular Component
Golgi apparatus
endoplasmic reticulum
Biological Context
Biological Process
ER to Golgi vesicle-mediated transport
Prabuddha Sengupta
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence microscopy
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
unprocessed raw data
Sample Preparation
unfixed tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px 0.11µm
Y 512px 0.11µm
Z 8px 1µm