Description

Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart) Timepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart) Timepoint3: 238 seconds (11 Z-slices at 1 um apart) Timepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point) Images were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals.