The Cell: An Image Library

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

Comments:

Add to Photobox

CIL:9070^*

Description

NRK cells expressing a mitochondria marker, mito-RFP (red), and PSS1-CFP (green), phosphatidylserine synthase 1. A single 6 micron confocal slice was imaged on a Zeiss LSM 510 every 5.9 sec with the follow parameters: Green: Ex: 458 nm laser, Em: Long Pass 475nm, Red: 543 nm laser, Em: Long pass 560 nm).

Biological Sources

NCBI Organism Classification: Rattus; (Old World rats)
Cell Type: fibroblast
Cell Line: NRK; Kidney
Cellular Component: mitochondrion; phosphatidylserine synthase 1

Biological Context

Biological Process: mitochondrion organization

Attribution

Name: Kasturi Mitra

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: mRFP1; ECFP
Processing History: unprocessed raw data; color combine

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.07μm
Y	512px	0.07μm
Channel	Wavelength
1	488, 543 nm
Time	5.9 sec

*CIL – Cell Image Library accession number. Please use this to reference an image.