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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9068*  Cite 

Saos2 cell line immunostained with Tom20 (mitochondrial outer membrane protein, green) and DLP1 (dynamin like protein, red) antibody. Cell population synchronized at G1-S transition of the cell cycle. This Saos2 cell line was serum starved (0.1%) for 3 days and released in released in aphidicolin (0.25 ug/ml) for 16 hours. The image is a single 3 micron confocal section collected on a Zeiss LSM 510 with the following parameters: Green: Ex: 488 nm laser, Em: Band Pass 500-550nm, Red: 543 nm laser, Em: Long pass 560 nm.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
Cell Line
Cellular Component
mitochondrial outer membrane
dynamin like protein
Biological Context
Biological Process
G1/S transition of mitotic cell cycle
Kasturi Mitra
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
color combine
Data Qualifiers
raw, unprocessed data
Sample Preparation
chemically fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px 0.29µm
Y 512px 0.29µm
Channel Wavelength
1 488, 543nm