The Cell: An Image Library

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CIL:8951^*

Description

Individual Mouse Aortic Endothelial Cell (MAEC) migrating in a 3-dimensional collagen gel (1.4mg/mL). Imaged on a spinning disk confocal microscope using a 100x oil immersion objective lens on a Nikon Eclipse Ti equipped with Perfect Focus System, an electronic shutter (Smart shutter, Sutter Instruments; Novato, CA) for transmitted illumination, a linear-encoded X, Y, Z robotic stage with a piezo-driven Z-axis top plate (ASI Technologies, Montgomeryville, PA) and a Yokogawa CSU-X spinning disk confocal scan head in an electronic filterwheel. Total imaging time = 1hr, 12mins.

Biological Sources

NCBI Organism Classification: Mus musculus; (house mouse)
Cell Type: blood vessel endothelial cell; primary cell line cell
Cellular Component: cell; extracellular matrix

Biological Context

Biological Process: cell migration

Attribution

Names: Kenneth Myers; Clare M. Waterman
Other: Laboratory of Cell and Tissue Morphodynamics, NHLBI, NIH

Imaging

Image Type: recorded image
Imaging Mode: phase contrast microscopy; spinning disk confocal microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro; cells in 3D matrix

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1392px	105nm
Y	1040px	105nm
Time	1 hr 12 min sec

*CIL – Cell Image Library accession number. Please use this to reference an image.