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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:843*  Cite 
Description

CFP-expressing neuron, 14 days in vitro. By two weeks, cultured hippocampal neurons have developed a morphology typical of mature neurons with an extensive axonal arbor, a tapered and branched dendritic arbor, and synaptic connections. Growth cones can be seen at the tips of axonal branches, and the dendritic arbor is studded with spines, specialized structures commonly associated with excitatory synapses. This image accompanies the second file in this image group which shows the fluorescent image. Embryonic rat hippocampal neurons were prepared and transfected with soluble CFP (excitation, 439; emission, 476) at plating (Dotap, Roche) as previously described (Kaech and Banker, 2006, Nat Protoc), fixed for 15 minutes (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4, warmed to 37°C, permeabilized with 0.25% Triton X-100 for 7 minutes, and rinsed with phosphate buffered saline at 14 days in vitro (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Images were acquired with a Leica DMRXA microscope with a mercury arc lamp, a 16X PL Fluotar (0.5 NA) oil immersion lens, Princeton Instruments Micromax CCD camera and MetaMorph software, using a Leica CFP filter cube. Linked images show: A) CFP expression throughout the extent of the neuron; B) a merged image of the fluorescent cell with respect to the phase image. This image was generated with the MetaMorph overlay function, and the single plane fluorescence view is included in this image group.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
multipolar neuron
Cellular Component
axon
dendrite
neuron spine
growth cone
Attribution
Names
Withers, Ginger
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL843
Archival Resource Key (ARK)
ark:/b7295/w9cil843
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
phase contrast microscopy
Parameters Imaged
fluorescence emission
refractive index
Source of Contrast
Accumulation of fluorescent probe
in-focus phase contrast
Visualization Methods
Cyan fluorescent proteins from Aequorea
Sample Preparation
Methods
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1300px 0.42µm
Y 1030px 0.42µm