During hippocampal neuron development in vitro, N-cadherin becomes selectively localized to excitatory synapses. This image shows immunolocalization of the NMDA receptor, which is postsynaptic. Other images in this image group show immunolocalization of N-cadherin, colocalized at synapses that are also immunopositive for the presynaptic glutamate transporters V-glut 1 and 2. Individual fluorescent images were acquired using a Nikon Diaphot 300 with a 60X objective. Preparation was fixed in 4% paraformaldehyde and permeabilized with .01% TritonX-100.
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