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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:807*  Cite 

During hippocampal neuron development in vitro, N-cadherin becomes selectively localized to excitatory synapses. This image shows immunolocalization of N-cadherin. Other images in this image group show this staining is colocalized at synapses that are also immunopositive for the presynaptic glutamate transporters V-glut 1 and 2 and for the postsynaptic NMDA receptor. Individual fluorescent images were acquired using a Nikon Diaphot 300 with a 60X objective. Preparation was fixed in 4% paraformaldehyde and permeabilized with .01% TritonX-100.

Biological Sources
NCBI Organism Classification
Cell Type
multipolar neuron
Cellular Component
excitatory synapse
synapse part
Tonya Anderson
Deanna Benson
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1280px 0.113µm
Y 1024px 0.113µm