Description

This animation shows a nucleosome located in its putative binding site on the SWI-SNF remodeling complex from yeast. The atomic structure of the DNA component of the nucleosome based on x-ray diffraction (red and green) is shown, together with the 3D envelope of SWI-SNF derived from cryo electron microscopy (gold). The location was inferred from crosslinking and other studies (see the PMID reference). The animation shows rotation about an arbitrary vertical axis. Rotation about the horizontal axis is shown in the grouped animation.

Technical Details

SWI-SNF structure was obtained from TEM images of material negatively stained with uranyl acetate, single particle reconstruction techniques used to derive the 3D volume, which was then thresholded to provide a surface representation. Different angular views of the 3D surface were used to create the movie. See: Smith CL et al. 2003 Structural analysis of the yeast SWI/SNF chromatin remodeling complex. Nat Struct Biol.10:141-5.