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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7806*  Cite 
Description

This animation shows a nucleosome located in its putative binding site on the SWI-SNF remodeling complex from yeast. The atomic structure of the DNA component of the nucleosome based on x-ray diffraction (red and green) is shown, together with the 3D envelope of SWI-SNF derived from cryo electron microscopy (gold). The location was inferred from crosslinking and other studies (see the PMID reference). The animation shows rotation about an arbitrary horizontal axis. Rotation about the vertical axis is shown in the grouped animation.

Technical Details

SWI-SNF structure was obtained from TEM images of material negatively stained with uranyl acetate, single particle reconstruction techniques used to derive the 3D volume, which was then thresholded to provide a surface representation. Different angular views of the 3D surface were used to create the movie. See: Smith CL et al. 2003 Structural analysis of the yeast SWI/SNF chromatin remodeling complex. Nat Struct Biol.10:141-5.

Biological Sources
NCBI Organism Classification
saccharomyces cerevisiae
Cellular Component
nucleosome remodeling complex
SWI/SNF complex
Biological Context
Biological Process
nucleosome positioning
chromatin remodeling
chromosome organization
Molecular Function
transcriptional regulation
ATP-dependent chromatin remodeler
chromatin binding
Attribution
Names
Chris Woodcock
Craig Peterson
Rachel Horowitz-scherer
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7806
Archival Resource Key (ARK)
ark:/b7295/w9cil7806
Grouping This image is part of a group.
Imaging
Image Type
animation
color graphic
Processing History
Animation based on 3D envelope
Dimensions
Spatial Axis Image Size Pixel Size
X 800px 0.1nm
Y 720px 0.1nm