The Cell: An Image Library

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CIL:7600^*

Description

High resolution transmission electron micrograph of Halteria showing both the large macronucleus with nuclear pores, dense heterochromatin bodies and nucleolus, and the small micronucleus.

Technical Details

TEM taken on 3/12/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 13,750X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Halteria grandinella
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Carolina Biological Supply Co., NC, U.S.A.
Cellular Component: macronucleus; micronucleus; nucleolus; nuclear pore; nuclear chromatin

Biological Context

Biological Process: macronuclear organization; micronuclear organization; nucleolar organization; nuclear pore distribution; nucleus organization

Attribution

Name: Richard Allen
Link: http://www5.pbrc.hawaii.edu/allen/

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4623px	1.1nm
Y	5114px	1.1nm

*CIL – Cell Image Library accession number. Please use this to reference an image.