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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7439*  Cite 
Description

Mouse Embryonic Fibroblasts (MEFs) grown on glass coverslips coated with 10 ug/ml Fibronectin. After 24 hrs cells were fixed in 3% paraformaldehyde and stained with mouse antibody to Paxillin, rabbit antibody to cortactin, followed by anti-mouse Alexa 561 goat antibody for Cortactin (cblue), anti-rabbit Alexa 647 for Paxillin (green) and Alexa 488 phalloidin (red) to stain for F-actin. Images were collected on a Nikon Eclipse imaged with a cooled charge coupled device (CCD; Orca II; Hamamatsu Photonics) using a 60x 1.4 NA Plan Apo PH objective lens (Nikon). Individual exposure times were used for each channel: 488: 200ms, 568: 200 ms, 647: 50 ms.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
primary cell line cell
Cellular Component
focal adhesion
actin cytoskeleton
Biological Context
Biological Process
cell adhesion
actin filament polymerization
Molecular Function
actin binding
cell adhesion molecule binding
Attribution
Names
Ana M. Pasapera
Clare M. Waterman
Pubmed
20308429
OTHER
NHLBI/ NIH
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7439
Archival Resource Key (ARK)
ark:/b7295/w9cil7439
Sample Preparation
Methods
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1392px 0.107nm
Y 1040px 0.107µm
Channel Wavelength
1 488nm
2 568nm
3 647nm


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