Description

Human foreskin fibroblasts (HFF1) cells transfected with mKusabira-Orange EB3, a marker for growing microtubule ends. Note the dramatic movement of the bright fluorescent markers toward the cell periphery. Cells were imaged every 3 s by a spinning disk confocal microscoe on a Nikon Ti2000 using a 1.4NA Plan Apo Ex objective and a solid state 568 laser with a 605x52nm EM filter (Semrock). Images were recorded with a Coolsnap HQ2 (Photometrics) using 500 ms exposures.