The Cell: An Image Library

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CIL:7354^*

Description

Newt (Notopthalmus viridescens erythrocytes were isolated in 0.1M KCl, spread on the surface of Na-citrate, picked up on formvar-carbon films, fixed with glutaraldehyde and negatively stained with 2% uranyl acetate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 45 degrees. A grouped image of the same field at a 55 degree tilt provides an oblique stereo view of a stretched tangle of chromatin fibers from the dispersed nucleus.

Biological Sources

NCBI Organism Classification: notopthalmus viridescens
Cell Type: erythrocyte
Cellular Component: nuclear chromatin

Biological Context

Biological Process: DNA packaging

Attribution

Name: Hans Ris
Pubmed: 7022124

Grouping

This image is part of a group.

Imaging

Image Type: film
Imaging Mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: negative staining; uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: spread nucleus

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3777px	0.5nm
Y	2798px	0.5nm

*CIL – Cell Image Library accession number. Please use this to reference an image.