Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7338^* Cite

Description

High resolution transmission electron micrograph of a portion of the macronucleus of Opercularia coarctata containing irregularly shaped heterochromatin bodies and elliptical nucleoli. Ribosome-like precursors can be seen at the margins of the nucleoli.

Technical Details

TEM taken on 6/11/69 by R. Allen with Philips 300 operating at 60kV. Neg. 23,200X. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Opercularia [NCBITaxon:168247]; Opercularia coarctata
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component: macronucleus; nuclear pore; nucleolus; heterochromatin; nuclear envelope

Biological Context

Biological Process: nucleolus organization; nucleus organization; chromatin organization

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7338
Archival Resource Key (ARK): ark:/b7295/w9cil7338

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3699px	0.86nm
Y	4520px	0.86nm