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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7334*  Cite 
Description

High resolution image of the contractile vacuole in diastole (filling phase). Decorated spongiome surrounds the CV. The macronucleus, micronucleus, cytopharynx, and basal bodies of perioral membranelle are included. TEM taken on 6/10/69 by R. Allen with Philips 300 operating at 60kV. Neg. 11,800X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Opercularia [NCBITaxon:168247]
Opercularia coarctata
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component
contractile vacuole
macronucleus
micronucleus
microtubule basal body
Biological Context
Biological Process
cv diastole
micronucleus organization
Molecular Function
macronucleus organization
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7334
Archival Resource Key (ARK)
ark:/b7295/w9cil7334
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 5521px 1.3nm
Y 3757px 1.3nm