The Cell: An Image Library

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CIL:7329^*

Description

Primary endothelial cells from transgenic mouse expressing myosin heavy chain 2A fused to mCherry (red), transfected with GFP-myosin (green) regulatory light chain expression vector and demonstrate the role of myosin in cell division. Cells are embedded in 3D collagen gels. mage Name: myosin cell division timelapse Biological Source: Primary mouse (mus musculus) aortic endothelial cell. 2D images over time. 0.1274 micron/pixel, 520x490 pixels, 20 seconds interval between timepoints.Time shown is in minutes:seconds. Images collected with a spinning disk confocal on a Nikon TE1000 with a 60X 1.2NA water objective using 400 ms exposure for mCherry and 200 ms for GFP. See Fischer et al for details regarding preparation. http://www.ncbi.nlm.nih.gov/pubmed/19185493

Biological Sources

NCBI Organism Classification: Mus musculus; (house mouse)
Cell Type: blood vessel endothelial cell; primary cell line cell
Cellular Component: myosin II complex

Biological Context

Biological Process: cell division; actomyosin contractile ring contraction
Molecular Function: myosin II binding

Attribution

Names: Robert S. Fischer; Clare M. Waterman
Pubmed: 19185493
Other: NHLBI/NIH

Imaging

Image Type: recorded image
Imaging Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; mCherryFP
Processing History: unprocessed raw data

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: dispersed cells in vitro; cells in 3D matrix

Dimensions

Spatial Axis	Image Size	Pixel Size
X	520px	.1274μm
Y	490px	.1274μm
Channel	Wavelength
1	488, 561 nm
Time	20 sec

*CIL – Cell Image Library accession number. Please use this to reference an image.