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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:731*  Cite 
Description

COS 7 cell expressing Organelle LightsTM Peroxi-GFP (Invitrogen)consisting of the peroxisomal C-terminal targeting sequence. Images were collected with a 63X 1.4 NA Plan-Apochromat oil objective on a Zeiss LSM510 META (Carl Zeiss, Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter. Image size is 512 x 512, pixel size is 0.14 µm x 0.14 µm and pixel time was set to 1.6 µsec. Detector pinhole was set to collect 300 ~0.5 µm slices at ~1 sec intervals

Biological Sources
NCBI Organism Classification
Chlorocebus sabaeus
Cell Line
COS-7
Cellular Component
peroxisome
Biological Context
Biological Process
peroxisome organization
Attribution
Names
George H. Patterson
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL731
Archival Resource Key (ARK)
ark:/b7295/w9cil731
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
EGFP
Visualization Methods
UV/visible/IR photons
Sample Preparation
Methods
living tissue
Relation To Intact Cell
living tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.1428µm
Y 512px 0.1428µm
Time 1 seconds 300