NG108-15 cells expressing EGFP-actin. NG108-15 cells are a cell line that is derived from a mouse neuroblastoma and a rat glioma. This is one of two images related images that show actin dynamics in the growth cones of the NG108 cells. This image captures the actin dynamics of multiple processes on the NG108 and the related image shows a more detailed view of filopodial dynamics in the growth cone. Images were collected as a single z slice time series on a Carl Zeiss 5 LIVE microscope. The Zeiss 5 LIVE uses a cylindrical lense to create a sheet of illumination that is rapidly scanned in the vertical direction and detected on a linear CCD array to enable faster imaging. The images were collected with a 63X 1.4 NA plan apo objective. Filers: NFT 490 and BP 500-525. 28 micron slit width. Pixel dwell 972 micros yielded approximately 2 frames collected per s.
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