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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7248*  Cite 

NG108-15 cells expressing EGFP-actin. NG108-15 cells are a cell line that is derived from a mouse neuroblastoma and a rat glioma. This is one of two images related images that show actin dynamics in the growth cones of the NG108 cells. This image captures the actin dynamics of multiple processes on the NG108 and the related image shows a more detailed view of filopodial dynamics in the growth cone. Images were collected as a single z slice time series on a Carl Zeiss 5 LIVE microscope. The Zeiss 5 LIVE uses a cylindrical lense to create a sheet of illumination that is rapidly scanned in the vertical direction and detected on a linear CCD array to enable faster imaging. The images were collected with a 63X 1.4 NA plan apo objective. Filers: NFT 490 and BP 500-525. 28 micron slit width. Pixel dwell 972 micros yielded approximately 2 frames collected per s.

Biological Sources
NCBI Organism Classification
mouse/rat NG108-15
Cell Line
Cellular Component
growth cone
Biological Context
Biological Process
axon guidance
Molecular Function
actin binding
Galbraith, Catherine
Galbraith, James
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
slit-scan confocal
Parameters Imaged
fluorescence emission
Source of Contrast
UV/visible/IR photons
Visualization Methods
Processing History
unprocessed raw data
Sample Preparation
unfixed tissue
Relation To Intact Cell
living tissue
Spatial Axis Image Size Pixel Size
X 330px 0.208µm
Y 400px 0.208µm
Time 0.5 seconds 110