HeLa cell in cytokinesis was fixed and stained for microtubules (green), RacGAP1 (red), and DNA (blue). Cells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against RacGAP1 (Rac GTPase-activating protein 1), TOPRO3 (Invitrogen) for DNA, and FITC-DM1a antibody against microtubules. Secondary antibody was Alexa 594 for RacGAP1. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an Orca ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, 568 Laser/ATOF 605/52, and 647 Laser ATOF 700/75.
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