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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7245*  Cite 
Description

HeLa cell in cytokinesis was fixed and stained for microtubules (green), RacGAP1 (red), and DNA (blue). Cells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against RacGAP1 (Rac GTPase-activating protein 1), TOPRO3 (Invitrogen) for DNA, and FITC-DM1a antibody against microtubules. Secondary antibody was Alexa 594 for RacGAP1. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an Orca ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, 568 Laser/ATOF 605/52, and 647 Laser ATOF 700/75.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
Cell Line
HeLa
Cellular Component
microtubule
nucleus
Biological Context
Biological Process
cytokinesis
Molecular Function
Rac GTPase binding
Attribution
Names
Hu, Chi-Kuo
OTHER
Tim Mitchison Lab
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7245
Archival Resource Key (ARK)
ark:/b7295/w9cil7245
Grouping This image is part of a group.
Sample Preparation
Methods
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px 0.064µm
Y 1024px 0.064µm
Channel Wavelength
1 488nm
2 568nm
3 647nm