The Cell: An Image Library

Licensing

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CIL:7224^*

Description

Distribution of Golgi Apparatus during cell division. Hela cells fixed with MeOH on ice for 3 min, and stained with a primary antibody against the Golgi and FITC conjugated DM1a antibody (green) against microtubules. Secondary antibody was Alexa 594 for the Golgi (red). A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an Orca ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, and 568 Laser/ATOF 605/52.

Biological Sources

NCBI Organism Classification: Homo sapiens; (human)
Cell Type: epithelial cell
Cell Line: HeLa
Cellular Component: Golgi apparatus; microtubule

Biological Context

Biological Process: cell division

Attribution

Name: Hu, Chi-Kuo
Other: Tim Mitchison Lab

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: primary antibody plus labeled secondary antibody; Alexa Fluor 594; Fluorescein (FITC); labeled primary antibody
Processing History: unprocessed raw data

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	0.064μm
Y	1024px	0.064μm
Channel	Wavelength
1	488 nm
2	568 nm

*CIL – Cell Image Library accession number. Please use this to reference an image.