TEM image showing the basic nucleosomal organization of eukaryotic chromatin. Beaded chains of nucleosomes separated by linker DNA are seen emanating from a central mass of less dispersed chromatin.
Chicken erythrocyte nuclei were allowed to decondense in a low salt high pH buffer, fixed with HCOH, centrifuged onto a carbon coated EM grid, and stained with uranyl acetate. (Spreading technique of Oscar L Miller Jr).
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