The Cell: An Image Library

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CIL:708^*

Description

chicken erythrocyte nuclei were allowed to decondense in a low salt high pH buffer, fixed with HCOH, centrifuged onto a carbon coated EM grid, and stained with uranyl acetate. (Spreading technique of Oscar L Miller Jr). Beaded chains of nucleosomes separated by linker DNA are seen emanating from a central mass of less dispersed chromatin.

Biological Sources

NCBI Organism Classification: Gallus gallus; (red junglefowl)
Cell Type: nucleate erythrocyte
Cellular Component: chromatin

Biological Context

Biological Process: DNA packaging
Molecular Function: structural constituent of chromatin

Attribution

Name: Chris Woodcock

Imaging

Image Type: transmission electron microscopy (TEM)
Imaging Mode: illumination by electrons
Parameters Imaged: electron density
Source of Contrast: elastic scattering
Visualization Methods: visualization of contiguous regions; uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4096px	0.73nm
Y	2804px	0.73nm

*CIL – Cell Image Library accession number. Please use this to reference an image.