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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:571*  Cite 
Description

At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation. Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium is being divided into uninuclear gametes, and the formation of flagella from paired centrioles is in progress. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319.

Technical Details

Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate. Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film.

Biological Sources
NCBI Organism Classification
Acetabularia mediterranea
Cell Type
gamete
Cellular Component
nucleus
chloroplast
plastid thylakoid membrane
chloroplast starch grain
Biological Context
Biological Process
gamete generation
Attribution
Names
Chris Woodcock
Gayle Miller
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL571
Archival Resource Key (ARK)
ark:/b7295/w9cil571
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 4040px 1.7nm
X 2810px 1.7nm