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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:54856*  Cite 

Lipid droplet contacts with nucleus and kinetoplast in a Leishmania amastigote inside golden hamster spleen cell. The image shows a transversal section of the cell. Below plasma membrane is possible to identify subpellicular microtubules. The lipid droplet is in contact with nuclear envelope and mitochondrial outer membrane. At the kinetoplast it is possible identify the mitochondrial DNA (kDNA) and mitochondrial outer and inner membranes. Golgi apparatus and endosomes also can be observed. Leishmania infantum amastigote form (MCAN/BR/89/BA262) observed in spleen section during experimental infection of Mesocricetus auratus. Tissue was fixed using karnovsky reagent, post-fixed with osmium tetroxide, dehydrated in ethanol and embedded in epoxy resin. Thin sections were viewed on a Zeiss 109 transmission electron microscope. Magnification 80000.

Technical Details

* Karnovsky fixed tissue
* Osmium tetroxide fixed tissue
* Tissue in epoxy resin embedment

Biological Sources
NCBI Organism Classification
Leishmania infantum
Cellular Component
golgi aparatus
mitochondrial DNA
mitochondrial outer membrane
mitochondrial inner membrane
subpelicular microtubules
lipid droplet
Biological Context
Biological Process
intracellular amastigote
Théo Araújo-Santos
Valéria M. Borges
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Mode
transmission electron microscopy (TEM)
Visualization Methods
osmium tetroxide