Alternate header for print version

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:54660*  Cite 

Confocal image of the CNS of a control D. melanogaster in the third instar larval stage, 70uM from the ventral surface, depicting astrocytes (red), cortex glia (green) and neuronal nuclei (blue). Our study examined the breakdown of the spatial segregation between cortex glial and astrocytes after disruption of cortex glial morphology. In a control animal, there is a separation between the two glial subtypes.

Technical Details

Astrocyte-specific RFP expression was achieved using driven using the Gal4-UAS binary system and the driver alrm. Cortex-glia specific GFP was achieved using the lexA-lexAop binary system and the cortex-glia specific driver wrapper932i-LexA (Coutinho-Budd et al., 2017). The larval CNS was dissected in the third instar larval stage, fixed with methanol, stained for RFP, GFP and Elav (neuronal nuclei marker), and mounted in VectaShield reagent. Slides were imaged on an Innovative Imaging Innovations (3i) spinning disk confocal microscope equipped with a Yokogawa CSX-W1 using a 63X oil objective.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
astrocytes (red)
cortex glia (green)
neurons (blue)
Cellular Component
Astrocyte membranes
cortex glia membranes
neuronal nuclei
Biological Context
Biological Process
Glial-Glial Tiling
Gabriela Salazar Lopez
Grace Ross
Ariana Maserejian
Jaeda Coutinho-Budd
6L.63X.WT 8.31.19 488Ctx Cy3ast Cy5elav 3B_6
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Mode
fluorescence microscopy
Visualization Methods
Spinning Disk Confocal Imaging