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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:51301*  Cite 
Description

A novel cell population analysis to quantify both initial and long-term drug effects, namely pseudopod adaptation and recovery following an initial drug-induced perturbation, adding drug slowly in 1/4 increments over 90 minutes.

Technical Details

~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C

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Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
RAW 264.7 TIB-71
Cellular Component
whole cell
Biological Context
Biological Process
Cell polarity establishment & maitenance
Attribution
Names
Thomas Buckles
OTHER
Population_Timecourses_fast
W9CILP20451
GO6976
120518
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL51301
Archival Resource Key (ARK)
ark:/b7295/w9cil51301
Grouping This image is part of a group.
Imaging
Image Mode
DICM
Visualization Methods
Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective