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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:50917*  Cite 
Description

Wild type Caenorhabditis elegans (N2 background) were high pressure frozen and visualized through transmission electron microscopy (TEM). The images show the nucleus of intestine cells and Oocytes and the black arrow indicates nuclear envelope budding events.

Technical Details

Worms were cultured onto normal growing medium plates spread with Escherichia coli (OP50 strain) and high pressure frozen in a Wohlwend Compact 3 machine, followed by a short freeze substitution protocol in 2% Uranyl acetate and embedded in HM20 resin. Sections of 70 nm thickness were then contrast stained with 2% Uranyl acetate and Reynold's lead citrate. Pictures were aquired in a Tecnai T12 TEM with a Ceta CMOS 16M camera.

Biological Sources
NCBI Organism Classification
Caenorhabditis elegans (roundworm, nematode)
Cell Type
Intestine cells and Oocytes
Cellular Component
nucleus
Biological Context
Biological Process
Nuclear envelope budding
Attribution
Names
Johanna Höög
Dimitra Panagaki
Jacob Croft
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL50917
Archival Resource Key (ARK)
ark:/b7295/w9cil50917
Grouping This image is part of a group.
Imaging
Image Mode
transmission electron microscopy (TEM)
Visualization Methods
Stain with Uranyl acetate and Lead citrate